Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics.

ABSTRACT: A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they were enriched around the terminus of replication. A quarter of the variable genes and two-thirds of the strain-specific genes were associated with mobile DNA. Signatures for horizontal gene transfer and modular

SUBMITTER: Berger B 

PROVIDER: S-EPMC1797336 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature