Streptococcus pneumoniae DivIVA: localization and interactions in a MinCD-free context.

ABSTRACT: To clarify the function of DivIVA in Streptococcus pneumoniae, we localized this protein in exponentially growing cells by both immunofluorescence microscopy and immunoelectron microscopy and found that S. pneumoniae DivIVA (DivIVA(SPN)) had a unique localization profile: it was present simultaneously both as a ring at the division septum and as dots at the cell poles. Double-immunofluorescence analysis suggested that DivIVA is recruited to the septum at a later stage than FtsZ and is retained at the poles after cell separation. All the other cell division proteins that we tested were localized in the divIVA null mutant, although the percentage of cells having constricted Z rings was significantly reduced. In agreement with its localization profile and consistent with its coiled-coil natur

SUBMITTER: Fadda D 

PROVIDER: S-EPMC1797354 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature