Global gene expression and phenotypic analysis of a Vibrio cholerae rpoH deletion mutant.

ABSTRACT: Vibrio cholerae, the cause of cholera, can grow in a variety of environments outside of human hosts. During infection, this pathogen must adapt to significant environmental alterations, including the elevated temperature of the human gastrointestinal tract. Sigma(32), an alternative sigma factor encoded by rpoH, activates transcription of genes involved in the heat shock response in several bacterial species. Here, we assessed the role of sigma(32) in V. cholerae physiology. In aggregate, our findings suggest that sigma(32) promotes V. cholerae growth at temperatures ranging at least from 15 degrees C to 42 degrees C. Growth of the rpoH mutant was severely attenuated within the suckling mouse intestine, suggesting that sigma(32)-regulated genes are critical for V. cholerae adaptation to co

SUBMITTER: Slamti L 

PROVIDER: S-EPMC1797412 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature