Epigenetic activation of a subset of mRNAs by eIF4E explains its effects on cell proliferation.

ABSTRACT:

Background

Translation deregulation is an important mechanism that causes aberrant cell growth, proliferation and survival. eIF4E, the mRNA 5' cap-binding protein, plays a major role in translational control. To understand how eIF4E affects cell proliferation and survival, we studied mRNA targets that are translationally responsive to eIF4E.

Methodology/principal findings

Microarray analysis of polysomal mRNA from an eIF4E-inducible NIH 3T3 cell line was performed. Inducible expression of eIF4E resulted in increased translation of defined sets of mRNAs. Many of the mRNAs are novel targets, including those that encode large- and small-subunit ribosomal proteins and cell growth-related factors. In addition, there was augmented translation of mRNAs encoding anti-apoptotic prote

SUBMITTER: Mamane Y 

PROVIDER: S-EPMC1797416 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature