The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells.

ABSTRACT: Recently a cell culture model supporting the complete life cycle of the hepatitis C virus (HCV) was developed. Searching for host cell determinants involved in the HCV replication cycle, we evaluated the efficiency of virus propagation in different Huh-7-derived cell clones. We found that Huh-7.5 cells and Huh7-Lunet cells, two former replicon cell clones that had been generated by removal of an HCV replicon by inhibitor treatment, supported comparable levels of RNA replication and particle production, whereas virus spread was severely impaired in the latter cells. Analysis of cell surface expression of CD81 and scavenger receptor class B type I (SR-BI), two molecules previously implicated in HCV entry, revealed similar expression levels for SR-BI, while CD81 surface expression was much hi

SUBMITTER: Koutsoudakis G 

PROVIDER: S-EPMC1797465 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature