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Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.

ABSTRACT: A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

SUBMITTER: Loftis AD 

PROVIDER: S-EPMC179802 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature

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Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.

Loftis Amanda D AD   Massung Robert F RF   Levin Michael L ML  

Journal of clinical microbiology 20030801 8

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis. ...[more]

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