Effect of human vasoactive intestinal peptide gene transfer in a murine model of Sjogren's syndrome.

ABSTRACT:

Background

Sjögren's syndrome (SS), an autoimmune exocrinopathy mainly affecting lachrymal and salivary glands, results in ocular and oral dryness (keratoconjunctivitis sicca and xerostomia). The aetiology and pathogenesis are largely unknown; currently, only palliative treatment is available.

Objective

To determine whether gene transfer of vasoactive intestinal peptide (VIP), based on its immunomodulatory properties, might be useful in the management of SS.

Methods

A recombinant serotype 2 adeno-associated virus encoding the human VIP transgene (rAAV2hVIP) was constructed and its efficacy tested in the female non-obese diabetic (NOD) mouse model for SS after retrograde instillation in submandibular glands (SMGs). 10(10) particles/gland of rAAV2hVIP or rAAV2LacZ (enco

SUBMITTER: Lodde BM 

PROVIDER: S-EPMC1798026 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature