Analysis of VEGF-responsive genes involved in the activation of endothelial cells.

ABSTRACT:

Background

Identification of the genes and pathways associated with the activation of endothelial cells (ECs) could help uncover the role of ECs in wound healing, vascular permeability, blood brain barrier function, angiogenesis, diabetic retinopathy, atherosclerosis, psoriasis, and growth of solid tumors.

Design

Herein, we embedded ECs in 3D type I collagen gel, left unstimulated or stimulated with VEGF165, and subjected to suppression subtractive hybridization followed by differential display (SSHDD). Gene fragments obtained from SSHDD were subjected to DNA sequence analysis. Database search with nucleotide sequence were performed using the BLAST algorithm and expression of candidate genes determined by northern blot analysis.

Results

A total of approximately 32 cDN

SUBMITTER: Wary KK 

PROVIDER: S-EPMC179881 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature