Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE).

ABSTRACT:

Background

The ADGE technique is a method designed to magnify the ratios of gene expression before detection. It improves the detection sensitivity to small change of gene expression and requires small amount of starting material. However, the throughput of ADGE is low. We integrated ADGE with DNA microarray (ADGE microarray) and compared it with regular microarray.

Results

When ADGE was integrated with DNA microarray, a quantitative relationship of a power function between detected and input ratios was found. Because of ratio magnification, ADGE microarray was better able to detect small changes in gene expression in a drug resistant model cell line system. The PCR amplification of templates and efficient labeling reduced the requirement of starting material to as little as

SUBMITTER: Chen ZJ 

PROVIDER: S-EPMC179896 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature