Alpha-tubulin minichromosome promoters in the stichotrichous ciliate Stylonychia lemnae.

ABSTRACT: Ciliated protists are model organisms for a number of molecular phenomena including telomerase function, self-splicing introns, and an RNA interference-related mechanism in programmed DNA elimination. Despite this relevance, our knowledge about promoters and transcriptional regulation in these organisms is very limited. The macronuclear genome of stichotrichous ciliates consists of minichromosomes which typically encode a single gene. The 5' nontranscribed spacers are usually no longer than 400 bp and highly suitable for promoter characterizations. We used microinjection of two artificial and differently tagged alpha1 tubulin minichromosomes into the macronucleus of Stylonychia lemnae as a means to characterize in detail the corresponding promoter. Clonal cell lines that stably maintained

SUBMITTER: Skovorodkin I 

PROVIDER: S-EPMC1800363 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature