Gbx2 and Otx2 interact with the WD40 domain of Groucho/Tle corepressors.

ABSTRACT: One of the earliest organizational decisions in the development of the vertebrate brain is the division of the neural plate into Otx2-positive anterior and Gbx2-positive posterior territories. At the junction of these two expression domains, a local signaling center is formed, known as the midbrain-hindbrain boundary (MHB). This tissue coordinates or "organizes" the development of neighboring brain structures, such as the midbrain and cerebellum. Correct positioning of the MHB is thought to depend on mutual repression involving these two homeobox genes. Using a cell culture colocalization assay and coimmunoprecipitation experiments, we show that engrailed homology region 1 (eh1)-like motifs of both transcription factors physically interact with the WD40 domain of Groucho/Tle corepressor pr

SUBMITTER: Heimbucher T 

PROVIDER: S-EPMC1800652 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature