Cytoplasmic decay of intergenic transcripts in Saccharomyces cerevisiae.

ABSTRACT: Eukaryotes produce a number of noncoding transcripts from intergenic regions. In Saccharomyces cerevisiae, such cryptic unstable transcripts (CUTs) are thought to be degraded in the nucleus by a process involving polyadenylation and 3'-to-5' degradation by the nuclear exosome. In this work, we examine the degradation pathway of the RNA SRG1, which is produced from an intergenic region and contributes to the regulation of the SER3 gene by promoter occlusion during SRG1 transcription. Although there is some effect on SRG1 transcript levels when the nuclear exosome is compromised, the bulk of the SRG1 RNA is degraded in the cytoplasm by decapping and 5'-to-3' exonucleolytic digestion. Examination of other CUTs suggests that individual CUTs can be degraded by a variety of different mechanisms,

SUBMITTER: Thompson DM 

PROVIDER: S-EPMC1800667 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature