Nutrient amendments in soil DNA stable isotope probing experiments reduce the observed methanotroph diversity.

ABSTRACT: Stable isotope probing (SIP) can be used to analyze the active bacterial populations involved in a process by incorporating 13C-labeled substrate into cellular components such as DNA. Relatively long incubation times are often used with laboratory microcosms in order to incorporate sufficient 13C into the DNA of the target organisms. Addition of nutrients can be used to accelerate the processes. However, unnatural concentrations of nutrients may artificially change bacterial diversity and activity. In this study, methanotroph activity and diversity in soil was examined during the consumption of 13CH4 with three DNA-SIP experiments, using microcosms with natural field soil water conditions, the addition of water, and the addition of mineral salts solution. Methanotroph population diversity

SUBMITTER: Cebron A 

PROVIDER: S-EPMC1800760 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature