Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5.

ABSTRACT: Early differentiation of B lymphocytes requires the function of multiple transcription factors that regulate the specification and commitment of the lineage. Loss- and gain-of-function experiments have provided important insight into the transcriptional control of B lymphopoiesis, whereby E2A was suggested to act upstream of EBF1 and Pax5 downstream of EBF1. However, this simple hierarchy cannot account for all observations, and our understanding of a presumed regulatory network, in which transcription factors and signaling pathways operate, is limited. Here, we show that the expression of the Ebf1 gene involves two promoters that are differentially regulated and generate distinct protein isoforms. We find that interleukin-7 signaling, E2A, and EBF1 activate the distal Ebf1 promoter, where

SUBMITTER: Roessler S 

PROVIDER: S-EPMC1800812 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature