A screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains.

ABSTRACT:

Background

Noncoding RNA species play a diverse set of roles in the eukaryotic cell. While much recent attention has focused on smaller RNA species, larger noncoding transcripts are also thought to be highly abundant in mammalian cells. To search for large noncoding RNAs that might control gene expression or mRNA metabolism, we used Affymetrix expression arrays to identify polyadenylated RNA transcripts displaying nuclear enrichment.

Results

This screen identified no more than three transcripts; XIST, and two unique noncoding nuclear enriched abundant transcripts (NEAT) RNAs strikingly located less than 70 kb apart on human chromosome 11: NEAT1, a noncoding RNA from the locus encoding for TncRNA, and NEAT2 (also known as MALAT-1). While the two NEAT transcripts share no sign

SUBMITTER: Hutchinson JN 

PROVIDER: S-EPMC1800850 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature