Efficient high-resolution deletion discovery in Caenorhabditis elegans by array comparative genomic hybridization.

ABSTRACT: We have developed array Comparative Genomic Hybridization for Caenorhabditis elegans as a means of screening for novel induced deletions in this organism. We designed three microarrays consisting of overlapping 50-mer probes to annotated exons and micro-RNAs, the first with probes to chromosomes X and II, the second with probes to chromosome II alone, and a third to the entire genome. These arrays were used to reliably detect both a large (50 kb) multigene deletion and a small (1 kb) single-gene deletion in homozygous and heterozygous samples. In one case, a deletion breakpoint was resolved to fewer than 50 bp. In an experiment designed to identify new mutations we used the X:II and II arrays to detect deletions associated with lethal mutants on chromosome II. One is an 8-kb deletion targe

SUBMITTER: Maydan JS 

PROVIDER: S-EPMC1800925 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature