High-resolution copy number analysis of paraffin-embedded archival tissue using SNP BeadArrays.

ABSTRACT: High-density SNP microarrays provide insight into the genomic events that occur in diseases like cancer through their capability to measure both LOH and genomic copy numbers. Where currently available methods are restricted to the use of fresh frozen tissue, we now describe the design and validation of copy number measurements using the Illumina BeadArray platform and the application of this technique to formalin-fixed, paraffin-embedded (FFPE) tissue. In fresh frozen tissue from a set of colorectal tumors with numerous chromosomal aberrations, our method measures copy number patterns that are comparable to values from established platforms, like Affymetrix GeneChip and BAC array-CGH. Moreover, paired comparisons of fresh frozen and FFPE tissues showed nearly identical patterns of genomic

SUBMITTER: Oosting J 

PROVIDER: S-EPMC1800928 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature