Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish.

ABSTRACT:

Background

Attempts to develop a mechanistic understanding of the effects of environmental estrogens on fish are increasingly conducted at the level of gene expression. Appropriate application of real-time PCR in such studies requires the use of a stably expressed 'housekeeping' gene as an internal control to normalize for differences in the amount of starting template between samples.

Results

We sought to identify appropriate genes for use as internal controls in experimental treatments with estrogen by analyzing the expression of eight functionally distinct 'housekeeping' genes (18S ribosomal RNA [18S rRNA], ribosomal protein l8 [rpl8], elongation factor 1 alpha [ef1a], glucose-6-phosphate dehydrogenase [g6pd], beta actin [bactin], glyceraldehyde-3-phosphate dehydrogenase

SUBMITTER: Filby AL 

PROVIDER: S-EPMC1802086 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature