Kinome profiling of Arabidopsis using arrays of kinase consensus substrates.

ABSTRACT:

Background

Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since substrates from many organisms are present we decided to test these arrays for the determination of kinase activities in the model plant species Arabidopsis thaliana.

Results

Kinome profiling using Arabidopsis cell extracts resulted in the labelling of many consensus peptides by kinases from the plant, indicating the usefulness of this kinome profiling tool for plants. Method development showed that fresh and frozen plant material could be used to make cell lysate

SUBMITTER: Ritsema T 

PROVIDER: S-EPMC1803769 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature