An evaluation of LSU rDNA D1-D2 sequences for their use in species identification.

ABSTRACT:

Background

Identification of species via DNA sequences is the basis for DNA taxonomy and DNA barcoding. Currently there is a strong focus on using a mitochondrial marker for this purpose, in particular a fragment from the cytochrome oxidase I gene (COI). While there is ample evidence that this marker is indeed suitable across a broad taxonomic range to delineate species, it has also become clear that a complementation by a nuclear marker system could be advantageous. Ribosomal RNA genes could be suitable for this purpose, because of their global occurrence and the possibility to design universal primers. However, it has so far been assumed that these genes are too highly conserved to allow resolution at, or even beyond the species level. On the other hand, it is known that ribosoma

SUBMITTER: Sonnenberg R 

PROVIDER: S-EPMC1805435 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature