Improved perfusion conditions for patch-clamp recordings on human erythrocytes.

ABSTRACT: Various configurations of the patch-clamp method are powerful tools for examining the transport of charged solutes across biological membranes. Originally developed for the study of relatively large cells which adhere to solid surfaces under in vitro culture, these methods have been increasingly applied to small cells or organelles in suspension. Under these conditions, a number of significant technical problems may arise as a result of the smaller geometry. Here, we examined these problems using human erythrocytes infected with the malaria parasite, Plasmodium falciparum, a system where experimental differences and the technical difficulty of erythrocyte patch-clamp have hindered universal agreement on the properties of the induced ion channels. We found that patch-clamp recordings on inf

SUBMITTER: Lisk G 

PROVIDER: S-EPMC1808333 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature