Stability of mRNA/DNA and DNA/DNA duplexes affects mRNA transcription.

ABSTRACT: Nucleic acids, due to their structural and chemical properties, can form double-stranded secondary structures that assist the transfer of genetic information and can modulate gene expression. However, the nucleotide sequence alone is insufficient in explaining phenomena like intron-exon recognition during RNA processing. This raises the question whether nucleic acids are endowed with other attributes that can contribute to their biological functions. In this work, we present a calculation of thermodynamic stability of DNA/DNA and mRNA/DNA duplexes across the genomes of four species in the genus Saccharomyces by nearest-neighbor method. The results show that coding regions are more thermodynamically stable than introns, 3'-untranslated regions and intergenic sequences. Furthermore, open rea

SUBMITTER: Kraeva RI 

PROVIDER: S-EPMC1808433 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature