Membrane localization of motility, signaling, and polyketide synthetase proteins in Myxococcus xanthus.

ABSTRACT: Myxococcus xanthus cells coordinate cellular motility, biofilm formation, and development through the use of cell signaling pathways. In an effort to understand the mechanisms underlying these processes, the inner membrane (IM) and outer membrane (OM) of strain DK1622 were fractionated to examine protein localization. Membranes were enriched from spheroplasts of vegetative cells and then separated into three peaks on a three-step sucrose gradient. The high-density fraction corresponded to the putative IM, the medium-density fraction corresponded to a putative hybrid membrane (HM), and the low-density fraction corresponded to the putative OM. Each fraction was subjected to further separation on discontinuous sucrose gradients, which resulted in discrete protein peaks for each major fraction

SUBMITTER: Simunovic V 

PROVIDER: S-EPMC181019 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature