Temporal dynamics of gene expression in the lung in a baboon model of E. coli sepsis.

ABSTRACT:

Background

Bacterial invasion during sepsis induces disregulated systemic responses that could lead to fatal lung failure. The purpose of this study was to relate the temporal dynamics of gene expression to the pathophysiological changes in the lung during the first and second stages of E. coli sepsis in baboons.

Results

Using human oligonucleotide microarrays, we have explored the temporal changes of gene expression in the lung of baboons challenged with sublethal doses of E. coli. Temporal expression pattern and biological significance of the differentially expressed genes were explored using clustering and pathway analysis software. Expression of selected genes was validated by real-time PCR. Cytokine levels in tissue and plasma were assayed by multiplex ELISA. Changes in

SUBMITTER: Zhu H 

PROVIDER: S-EPMC1819384 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature