Genes and proteins differentially expressed during in vitro malignant transformation of bovine pancreatic duct cells.

ABSTRACT: Pancreatic carcinoma has an extremely bad prognosis due to lack of early diagnostic markers and lack of effective therapeutic strategies. Recently, we have established an in vitro model recapitulating the first steps in the carcinogenesis of the pancreas. SV40 large T antigen-immortalized bovine pancreatic duct cells formed intrapancreatic adenocarcinoma tumors on k-ras(mut) transfection after orthotopic injection in the nude mouse pancreas. Here we identified genes and proteins differentially expressed in the course of malignant transformation using reciprocal suppression subtractive hybridization and 2D gel electrophoresis and mass spectrometry, respectively. We identified 34 differentially expressed genes, expressed sequence tags, and 15 unique proteins. Differential expression was veri

SUBMITTER: Jesnowski R 

PROVIDER: S-EPMC1819583 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature