CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wide.

ABSTRACT: CTCF is a transcription factor with highly versatile functions ranging from gene activation and repression to the regulation of insulator function and imprinting. Although many of these functions rely on CTCF-DNA interactions, it is an emerging realization that CTCF-dependent molecular processes involve CTCF interactions with other proteins. In this study, we report the association of a subpopulation of CTCF with the RNA polymerase II (Pol II) protein complex. We identified the largest subunit of Pol II (LS Pol II) as a protein significantly colocalizing with CTCF in the nucleus and specifically interacting with CTCF in vivo and in vitro. The role of CTCF as a link between DNA and LS Pol II has been reinforced by the observation that the association of LS Pol II with CTCF target sites in v

SUBMITTER: Chernukhin I 

PROVIDER: S-EPMC1820452 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature