Knockdown of ALR (MLL2) reveals ALR target genes and leads to alterations in cell adhesion and growth.

ABSTRACT: ALR (MLL2) is a member of the human MLL family, which belongs to a larger SET1 family of histone methyltransferases. We found that ALR is present within a stable multiprotein complex containing a cohort of proteins shared with other SET1 family complexes and several unique components, such as PTIP and the jumonji family member UTX. Like other complexes formed by SET1 family members, the ALR complex exhibited strong H3K4 methyltransferase activity, conferred by the ALR SET domain. By generating ALR knockdown cell lines and comparing their expression profiles to that of control cells, we identified a set of genes whose expression is activated by ALR. Some of these genes were identified by chromatin immunoprecipitation as direct ALR targets. The ALR complex was found to associate in an ALR-de

SUBMITTER: Issaeva I 

PROVIDER: S-EPMC1820476 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature