Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis.

ABSTRACT: Blastocyst formation during mammalian preimplantation development is a unique developmental process that involves lineage segregation between the inner cell mass and the trophectoderm. To elucidate the molecular mechanisms underlying blastocyst formation, we have functionally screened a subset of preimplantation embryo-associated transcripts by using small interfering RNA (siRNA) and identified Bysl (bystin-like) as an essential gene for this process. The development of embryos injected with Bysl siRNA was arrested just prior to blastocyst formation, resulting in a defect in trophectoderm differentiation. Silencing of Bysl by using an episomal short hairpin RNA expression vector inhibited proliferation of embryonic stem cells. Exogenously expressed Bysl tagged with a fluorescent protein wa

SUBMITTER: Adachi K 

PROVIDER: S-EPMC1820511 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature