Prion-induced amyloid heart disease with high blood infectivity in transgenic mice.

ABSTRACT: We investigated extraneural manifestations in scrapie-infected transgenic mice expressing prion protein lacking the glycophosphatydylinositol membrane anchor. In the brain, blood, and heart, both abnormal protease-resistant prion protein (PrPres) and prion infectivity were readily detected by immunoblot and by inoculation into nontransgenic recipients. The titer of infectious scrapie in blood plasma exceeded 10(7) 50% infectious doses per milliliter. The hearts of these transgenic mice contained PrPres-positive amyloid deposits that led to myocardial stiffness and cardiac disease.

SUBMITTER: Trifilo MJ 

PROVIDER: S-EPMC1820586 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature