Modulation of gene expression in a human cell line caused by poliovirus, vaccinia virus and interferon.

ABSTRACT:

Background

The project was initiated to describe the response of a human embryonic fibroblast cell line to the replication of two different viruses, and, more specifically, to look for candidate genes involved in viral defense. For this purpose, the cells were synchronously infected with poliovirus in the absence or presence of interferon-alpha, or with vaccinia virus, a virus that is not inhibited by interferon. By comparing the changes in transcriptosome due to these different challenges, it should be possible to suggest genes that might be involved in defense.

Results

The viral titers were sufficient to yield productive infection in a majority of the cells. The cells were harvested in triplicate at various time-points, and the transcriptosome compared with mock infected c

SUBMITTER: Grinde B 

PROVIDER: S-EPMC1821010 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature