A database of PCR primers for the chloroplast genomes of higher plants.

ABSTRACT:

Background

Chloroplast genomes evolve slowly and many primers for PCR amplification and analysis of chloroplast sequences can be used across a wide array of genera. In some cases 'universal' primers have been designed for the purpose of working across species boundaries. However, the essential information on these primer sequences is scattered throughout the literature.

Results

A database is presented here which assembles published primer information for chloroplast DNA. Additional primers were designed to fill gaps where little or no primer information could be found. Amplicons are either the genes themselves (typically useful in studies of sequence variation in higher-order phylogeny) or they are spacers, introns, and intergenic regions (for studies of phylogeographic patt

SUBMITTER: Heinze B 

PROVIDER: S-EPMC1821018 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature