Comparative analysis of ESTs involved in grape responses to Xylella fastidiosa infection.

ABSTRACT:

Background

The gram-negative bacterium Xylella fastidiosa (Xf) is the causal agent of Pierce's disease (PD) in grape as well as diseases of many fruit and ornamental plants. The current molecular breeding efforts have identified genetic basis of PD resistance in grapes. However, the transcriptome level characterization of the host response to this pathogen is lacking.

Results

Twelve tissue specific subtractive suppression hybridization (SSH) cDNA libraries derived from a time course sampling scheme were constructed from stems, leaves and shoots of PD resistant and susceptible sibling genotypes (V. rupestris x V. arizonica) in response to Xf infection. A total of 5,794 sequences were obtained from these cDNA libraries from which 993 contigs and 949 singletons were derived. Us

SUBMITTER: Lin H 

PROVIDER: S-EPMC1821027 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature