PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.

ABSTRACT: We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.

SUBMITTER: Schild M 

PROVIDER: S-EPMC1828997 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature