Transcriptional analysis of early lineage commitment in human embryonic stem cells.

ABSTRACT:

Background

The mechanisms responsible for the maintenance of pluripotency in human embryonic stem cells, and those that drive their commitment into particular differentiation lineages, are poorly understood. In fact, even our knowledge of the phenotype of hESC is limited, because the immunological and molecular criteria presently used to define this phenotype describe the properties of a heterogeneous population of cells.

Results

We used a novel approach combining immunological and transcriptional analysis (immunotranscriptional profiling) to compare gene expression in hESC populations at very early stages of differentiation. Immunotranscriptional profiling enabled us to identify novel markers of stem cells and their differentiated progeny, as well as novel potential regulat

SUBMITTER: Laslett AL 

PROVIDER: S-EPMC1829156 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature