Automated array-CGH optimized for archival formalin-fixed, paraffin-embedded tumor material.

ABSTRACT:

Background

Array Comparative Genomic Hybridization (aCGH) is a rapidly evolving technology that still lacks complete standardization. Yet, it is of great importance to obtain robust and reproducible data to enable meaningful multiple hybridization comparisons. Special difficulties arise when aCGH is performed on archival formalin-fixed, paraffin-embedded (FFPE) tissue due to its variable DNA quality. Recently, we have developed an effective DNA quality test that predicts suitability of archival samples for BAC aCGH.

Methods

In this report, we first used DNA from a cancer cell-line (SKBR3) to optimize the aCGH protocol for automated hybridization, and subsequently optimized and validated the procedure for FFPE breast cancer samples. We aimed for highest throughput, accuracy,

SUBMITTER: Joosse SA 

PROVIDER: S-EPMC1829401 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature