Nidogen 1 and 2 gene promoters are aberrantly methylated in human gastrointestinal cancer.

ABSTRACT:

Background

Nidogens are highly conserved proteins of basement membranes. Two nidogen proteins, nidogen 1 and nidogen 2, are known in mammals.

Results

We show that CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines. For both genes, methylation was correlated with loss of gene transcription in human cell lines. Furthermore, demethylation of the NID1 and NID2 promoters restored gene transcription, demonstrating that methylation was responsible for silencing nidogen genes. In primary tumors, we detected NID1 promoter methylation in 67% of colon cancer samples and in 90% of gastric cancers. NID2 promoter was methylated in 29% of colon and 95% of gastric cancers. Immuno-staining for nidogen-2 confirmed the correlation be

SUBMITTER: Ulazzi L 

PROVIDER: S-EPMC1831485 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature