Distinct glucose-dependent stress responses revealed by translational profiling in pancreatic beta-cells.

ABSTRACT: In pancreatic beta-cells, following an acute (within 1 h) increase in glucose concentration, there are rapid changes in the expression of a large subset of proteins. The change in the expression of many of these proteins is mediated by a post-transcriptional mechanism through either increases or decreases in the rate of translation from pre-existing transcripts. These proteins, whose synthesis is rapidly up- or down-regulated in response to glucose, are likely important in mounting the correct response to changes in plasma glucose concentrations. However, the vast majority of these proteins remain unidentified. Therefore, in order to identify these proteins, we analysed changes in the levels of mRNAs associated with polysomes (i.e. actively translating mRNAs) isolated from mouse insulinoma

SUBMITTER: Greenman IC 

PROVIDER: S-EPMC1831533 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature