Transcript specificity in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components.

ABSTRACT: Appropriate expression of most eukaryotic genes requires the removal of introns from their pre-messenger RNAs (pre-mRNAs), a process catalyzed by the spliceosome. In higher eukaryotes a large family of auxiliary factors known as SR proteins can improve the splicing efficiency of transcripts containing suboptimal splice sites by interacting with distinct sequences present in those pre-mRNAs. The yeast Saccharomyces cerevisiae lacks functional equivalents of most of these factors; thus, it has been unclear whether the spliceosome could effectively distinguish among transcripts. To address this question, we have used a microarray-based approach to examine the effects of mutations in 18 highly conserved core components of the spliceosomal machinery. The kinetic profiles reveal clear difference

SUBMITTER: Pleiss JA 

PROVIDER: S-EPMC1831718 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature