Ontology highlight
ABSTRACT:
SUBMITTER: Mori T
PROVIDER: S-EPMC1831719 | biostudies-literature | 2007 Apr
REPOSITORIES: biostudies-literature
Mori Tetsuya T Williams Dewight R DR Byrne Mark O MO Qin Ximing X Egli Martin M Mchaourab Hassane S HS Stewart Phoebe L PL Johnson Carl Hirschie CH
PLoS biology 20070401 4
A biochemical oscillator can be reconstituted in vitro with three purified proteins, that displays the salient properties of circadian (daily) rhythms, including self-sustained 24-h periodicity that is temperature compensated. We analyze the biochemical basis of this oscillator by quantifying the time-dependent interactions of the three proteins (KaiA, KaiB, and KaiC) by electron microscopy and native gel electrophoresis to elucidate the timing of the formation of complexes among the Kai protein ...[more]