Branch migration displacement assay with automated heuristic analysis for discrete DNA length measurement using DNA microarrays.

ABSTRACT: The analysis of short tandem repeats (STRs) plays an important role in forensic science, human identification, genetic mapping, and disease diagnostics. Traditional STR analysis utilizes gel- or column-based approaches to analyze DNA repeats. Individual STR alleles are separated and distinguished according to fragment length; thus the assay is generally hampered by its low multiplex capacity. However, use of DNA microarray would employ a simple hybridization and detection for field forensics and biology. Here we demonstrate a rapid, highly sensitive method for STR analysis that utilizes DNA microarray technology. We describe two adaptations to accomplish this: the use of competitive hybridization to remove unpaired ssDNA from an array and the use of neural network classification to automat

SUBMITTER: Pourmand N 

PROVIDER: S-EPMC1838402 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature