Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development.

ABSTRACT:

Background

Electroporation is a technique for the introduction of nucleic acids and other macromolecules into cells. In chick embryos it has been a particularly powerful technique for the spatial and temporal control of gene expression in developmental studies. Electroporation methods have also been reported for Xenopus, zebrafish, and mouse.

Results

We present a new protocol for zebrafish brain electroporation. Using a simple set-up with fixed spaced electrodes and microinjection equipment, it is possible to electroporate 50 to 100 embryos in 1 hour with no lethality and consistently high levels of transgene expression in numerous cells. Transfected cells in the zebrafish brain are amenable to in vivo time lapse imaging. Explants containing transfected neurons can be cultur

SUBMITTER: Hendricks M 

PROVIDER: S-EPMC1838412 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature