Model based analysis of real-time PCR data from DNA binding dye protocols.

ABSTRACT:

Background

Reverse transcription followed by real-time PCR is widely used for quantification of specific mRNA, and with the use of double-stranded DNA binding dyes it is becoming a standard for microarray data validation. Despite the kinetic information generated by real-time PCR, most popular analysis methods assume constant amplification efficiency among samples, introducing strong biases when amplification efficiencies are not the same.

Results

We present here a new mathematical model based on the classic exponential description of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The model was validated with experimental results and used for the development of a new method for real-time PCR data analysis. This model based method

SUBMITTER: Alvarez MJ 

PROVIDER: S-EPMC1838433 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature