An explicit test of the phospholipid saturation hypothesis of acquired cold tolerance in Caenorhabditis elegans.

ABSTRACT: Protection of poikilothermic animals from seasonal cold is widely regarded as being causally linked to changes in the unsaturation of membrane phospholipids, yet in animals this proposition remains formally untested. We have now achieved this by the genetic manipulation of lipid biosynthesis of Caenorhabditis elegans independent of temperature. Worms transferred from 25 degrees C to 10 degrees C develop over several days a much-increased tolerance of lethal cold (0 degrees C) and also an increased phospholipid unsaturation, as in higher animal models. Of the three C. elegans Delta9-desaturases, transcript levels of fat-7 only were up-regulated by cold transfer. RNAi suppression of fat-7 caused the induction of fat-5 desaturase, so to control desaturase expression we combined RNAi of fat-7

SUBMITTER: Murray P 

PROVIDER: S-EPMC1838478 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature