Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host.

ABSTRACT: Proteorhodopsins (PRs) are retinal-containing proteins that catalyze light-activated proton efflux across the cell membrane. These photoproteins are known to be globally distributed in the ocean's photic zone, and they are found in a diverse array of Bacteria and Archaea. Recently, light-enhanced growth rates and yields have been reported in at least one PR-containing marine bacterium, but the physiological basis of light-activated growth stimulation has not yet been determined. To describe more fully PR photosystem genetics and biochemistry, we functionally surveyed a marine picoplankton large-insert genomic library for recombinant clones expressing PR photosystems in vivo. Our screening approach exploited transient increases in vector copy number that significantly enhanced the sensitivi

SUBMITTER: Martinez A 

PROVIDER: S-EPMC1838496 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature