Allelotyping of pooled DNA with 250 K SNP microarrays.

ABSTRACT:

Background

Genotyping technologies for whole genome association studies are now available. To perform such studies to an affordable price, pooled DNA can be used. Recent studies have shown that GeneChip Human Mapping 10 K and 50 K arrays are suitable for the estimation of the allele frequency in pooled DNA. In the present study, we tested the accuracy of the 250 K Nsp array, which is part of the 500 K array set representing 500,568 SNPs. Furthermore, we compared different algorithms to estimate allele frequencies of pooled DNA.

Results

We could confirm that the polynomial based probe specific correction (PPC) was the most accurate method for allele frequency estimation. However, a simple k-correction, using the relative allele signal (RAS) of heterozygous individuals, perfor

SUBMITTER: Wilkening S 

PROVIDER: S-EPMC1839100 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature