Systematic identification of cellular signals reactivating Kaposi sarcoma-associated herpesvirus.

ABSTRACT: The herpesvirus life cycle has two distinct phases: latency and lytic replication. The balance between these two phases is critical for viral pathogenesis. It is believed that cellular signals regulate the switch from latency to lytic replication. To systematically evaluate the cellular signals regulating this reactivation process in Kaposi sarcoma-associated herpesvirus, the effects of 26,000 full-length cDNA expression constructs on viral reactivation were individually assessed in primary effusion lymphoma-derived cells that harbor the latent virus. A group of diverse cellular signaling proteins were identified and validated in their effect of inducing viral lytic gene expression from the latent viral genome. The results suggest that multiple cellular signaling pathways can reactivate th

SUBMITTER: Yu F 

PROVIDER: S-EPMC1839163 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature