Single-gene detection and karyotyping using small-target fluorescence in situ hybridization on maize somatic chromosomes.

ABSTRACT: Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive elements. In this study, repeat-free portions of sequences that were anchored to particular chromosomes including genes, gene clusters, large cDNAs, and portions of BACs obtained from public databases were used to label the corresponding physical location using FISH. A collection of probes that includes at least one marker on each chromosome in the maize complement was assembled, allowing a small-target

SUBMITTER: Lamb JC 

PROVIDER: S-EPMC1840074 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature