Rheb-TOR signaling promotes protein synthesis, but not glucose or amino acid import, in Drosophila.

ABSTRACT:

Background

The Ras-related GTPase, Rheb, regulates the growth of animal cells. Genetic and biochemical tests place Rheb upstream of the target of rapamycin (TOR) protein kinase, and downstream of the tuberous sclerosis complex (TSC1/TSC2) and the insulin-signaling pathway. TOR activity is regulated by nutritional cues, suggesting that Rheb might either control, or respond to, nutrient availability.

Results

We show that Rheb and TOR do not promote the import of glucose, bulk amino acids, or arginine in Drosophila S2 cells, but that both gene products are important regulators of ribosome biogenesis, protein synthesis, and cell size. S2 cell size, protein synthesis, and glucose import were largely insensitive to manipulations of insulin signaling components, suggesting that cel

SUBMITTER: Hall DJ 

PROVIDER: S-EPMC1847425 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature