Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue.

ABSTRACT: Formalin-fixed, paraffin-embedded tissue is the most widely available material for retrospective clinical studies. In combination with the potential of genomics, these tissues represent an invaluable resource for the elucidation of disease mechanisms and validation of differentially expressed genes as novel therapeutic targets or prognostic indicators. We describe here an approach that, in combination with laser-assisted microdissection allows quantitative gene expression analysis in formalin-fixed, paraffin-embedded archival tissue. Using an optimized RNA microscale extraction procedure in conjunction with real-time quantitative reverse transcriptase-polymerase chain reaction based on fluorogenic TaqMan methodology, we analyzed the expression of a panel of cancer-relevant genes, EGF-R, HE

SUBMITTER: Specht K 

PROVIDER: S-EPMC1850313 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature