Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.

ABSTRACT:

Background

The linkage between duplicated chromosomes (sister chromatids) is established during S phase by the action of cohesin, a multisubunit complex conserved from yeast to humans. Most cohesin dissociates from chromosome arms when the cell enters mitotic prophase, leading to the formation of metaphase chromosomes with two cytologically discernible chromatids. This process is known as sister-chromatid resolution. Although two mitotic kinases have been implicated in this process, it remains unknown exactly how the cohesin-mediated linkage is destabilized at a mechanistic level.

Results

The wings apart-like (Wapl) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. We show that the human or

SUBMITTER: Gandhi R 

PROVIDER: S-EPMC1850625 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature